ARG66551

anti-c-Myb antibody

anti-c-Myb antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes c-Myb
Tested Reactivity Hu, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name c-Myb
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of Human c-Myb.
Conjugation Un-conjugated
Alternate Names Transcriptional activator Myb; Proto-oncogene c-Myb; c-myb_CDS; c-myb; Cmyb; efg

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:500
IHC-P1:100 - 1:200
WB1:500 - 1:1000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 72 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
Preservative 0.01% Sodium azide
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4602 Human MYB

Swiss-port # P10242 Human Transcriptional activator Myb

Gene Symbol MYB
Gene Full Name v-myb avian myeloblastosis viral oncogene homolog
Background This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]
Function Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 72 kDa
PTM Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.

Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.

Phosphorylated by HIPK1. This phosphorylation reduces MYB transcription factor activity but not MYB protein levels. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG66551 anti-c-Myb antibody ICC/IF image

    Immunofluorescence: Formalin-fixed HeLa cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66551 anti-c-Myb antibody (red) in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. DAPI was used to stain the cell nuclei (blue).

  • ARG66551 anti-c-Myb antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then stained with ARG66551 anti-c-Myb antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • ARG66551 anti-c-Myb antibody WB image

    Western blot: K562 and Rat skeletal muscle lysates stained with ARG66551 anti-c-Myb antibody.