ARG51813
anti-eIF2 beta phospho (Ser2) antibody
anti-eIF2 beta phospho (Ser2) antibody for Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes eIF2 beta phospho (Ser2) |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | eIF2 beta |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 2 (M-S(p)-G-D-E) derived from Human eIF2β. |
| Conjugation | Un-conjugated |
| Alternate Names | EIF2beta; Eukaryotic translation initiation factor 2 subunit beta; eIF-2-beta; Eukaryotic translation initiation factor 2 subunit 2; EIF2; EIF2B; PPP1R67 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P20042 Human Eukaryotic translation initiation factor 2 subunit 2 Swiss-port # Q99L45 Mouse Eukaryotic translation initiation factor 2 subunit 2 |
|---|---|
| Gene Symbol | EIF2S2 |
| Gene Full Name | eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa |
| Background | eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
| Function | eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 38 kDa |
Images (1) Click the Picture to Zoom In
-
ARG51813 anti-eIF2 beta phospho (Ser2) antibody WB image
Western blot: Extracts from C2C12 cells untreated or treated with PMA stained with ARG51813 anti-eIF2 beta phospho (Ser2) antibody.
