ARG42704
anti-eIF2B alpha antibody
anti-eIF2B alpha antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes eIF2B alpha |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | eIF2B alpha |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to a sequence of Human eIF2B alpha. (AESFKFVRLFPLNQQD) |
| Conjugation | Un-conjugated |
| Alternate Names | Translation initiation factor eIF-2B subunit alpha; EIF2B; eIF-2B GDP-GTP exchange factor subunit alpha; EIF2BA |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | ~ 34 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.01% Sodium azide and 4% Trehalose. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EIF2B1 |
| Gene Full Name | eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa |
| Background | This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Oct 2009] |
| Function | Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. [UniProt] |
| Calculated MW | 34 kDa |
Images (5) Click the Picture to Zoom In
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ARG42704 anti-eIF2B alpha antibody ICC/IF image
Immunofluorescence: HeLa cells were blocked with 10% goat serum and then stained with ARG42704 anti-eIF2B alpha antibody (green) at 5 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42704 anti-eIF2B alpha antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42704 anti-eIF2B alpha antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG42704 anti-eIF2B alpha antibody WB image
Western blot: 50 µg of samples under reducing condition. Rat liver, Rat kidney and Mouse liver lysates stained with ARG42704 anti-eIF2B alpha antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42704 anti-eIF2B alpha antibody FACS image
Flow Cytometry: PC-3 cells were blocked with 10% normal goat serum and then stained with ARG42704 anti-eIF2B alpha antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG42704 anti-eIF2B alpha antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42704 anti-eIF2B alpha antibody at 2 µg/ml dilution, overnight at 4°C.
