Overview

Product Description	Rabbit Polyclonal antibody recognizes eIF3E
Tested Reactivity	Hu, Zfsh
Predict Reactivity	Ms, Rat, Bov, Chk, Mk, Xenopus
Tested Application	WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	eIF3E
Antigen Species	Human
Immunogen	KLH-conjugated synthetic peptide corresponding to aa. 248-276 of Human eIF3E.
Conjugation	Un-conjugated
Alternate Names	Viral integration site protein INT-6 homolog; EIF3-P48; EIF3S6; eIF3e; Eukaryotic translation initiation factor 3 subunit 6; eIF3-p46; INT6; Eukaryotic translation initiation factor 3 subunit E; eIF-3 p48

Application Instructions

Application Suggestion	Tested Application Dilution WB 1:1000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	K562

Properties

Form	Liquid
Purification	Purification with Protein A and immunogen peptide.
Buffer	PBS and 0.09% (W/V) Sodium azide.
Preservative	0.09% (W/V) Sodium azide.
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 3646 Human EIF3E Swiss-port # P60228 Human Eukaryotic translation initiation factor 3 subunit E
Gene Symbol	EIF3E
Gene Full Name	eukaryotic translation initiation factor 3, subunit E
Function	Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. [UniProt]
Cellular Localization	Cytoplasm. Nucleus, PML body. [UniProt]
Calculated MW	52 kDa

Images (1) Click the Picture to Zoom In