ARG65707
anti-eIF4G2 antibody
anti-eIF4G2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes eIF4G2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | eIF4G2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human eIF4G2 |
| Conjugation | Un-conjugated |
| Alternate Names | Eukaryotic translation initiation factor 4 gamma 2; eIF4G 2; eIF-4-gamma 2; DAP-5; NAT1; P97; Death-associated protein 5; DAP5; AAG1; eIF-4G 2; p97 |
Application Instructions
| Predict Reactivity Note | Rat | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | BT474 |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P78344 Human Eukaryotic translation initiation factor 4 gamma 2 Swiss-port # Q62448 Mouse Eukaryotic translation initiation factor 4 gamma 2 |
|---|---|
| Gene Symbol | EIF4G2 |
| Gene Full Name | eukaryotic translation initiation factor 4 gamma, 2 |
| Background | Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008] |
| Function | Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody |
| Calculated MW | 102 kDa |
| PTM | Phosphorylation; hyperphosphorylated during mitosis. |
Images (2) Click the Picture to Zoom In
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ARG65707 anti-eIF4G2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue stained with ARG65707 anti-eIF4G2 antibody.
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ARG65707 anti-eIF4G2 antibody WB image
Western blot: BT474 cell lysate stained with ARG65707 anti-eIF4G2 antibody.
