ARG23299
anti-gamma Actin antibody [2A3]
anti-gamma Actin antibody [2A3] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Mouse Monoclonal antibody [2A3] recognizes gamma Actin Mouse anti Human actin gamma antibody, clone 2A3 recognises cytoplasmic actin gamma (known also as ACTG and Gamma Actin), a 41. 8 kDa cytoskeletal protein. Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified (Vandekerckhove and Weber 1978) . Clone 2A3 is highly specific for actin gamma and does not react with other actin isoforms (Dugina et al. 2009). |
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| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2A3 |
| Isotype | IgG2b |
| Target Name | gamma Actin |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to the N-terminal nonapeptide of gamma-cytoplasmic actin. |
| Conjugation | Un-conjugated |
| Alternate Names | BRWS2; Actin, cytoplasmic 2; DFNA26; DFNA20; ACT; HEL-176; Gamma-actin; ACTG |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
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| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | ACTG1 |
| Gene Full Name | actin gamma 1 |
| Background | Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011] |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt] |
| Calculated MW | 42 kDa |
| PTM | The methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG23299 anti-gamma Actin antibody [2A3] WB image
Western blot: HeLa whole cell lysate stained with ARG23299 anti-gamma Actin antibody [2A3].
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ARG23299 anti-gamma Actin antibody [2A3] WB image
Western blot: NIH-3T3 murine embryonic fibroblast whole cell lysate stained with ARG23299 anti-gamma Actin antibody [2A3].
