ARG10759
anti-mCherry antibody [1C51]
anti-mCherry antibody [1C51] for ICC/IF,IHC-Frozen sections,Western blot and Other
Overview
| Product Description | Mouse Monoclonal Antibody to mCherry |
|---|---|
| Tested Reactivity | Other |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1C51 |
| Isotype | IgG2a |
| Target Name | mCherry |
| Antigen Species | Others |
| Immunogen | Full length recombinant protein. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification. |
| Buffer | PBS and 50% Glycerol. |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (3) Click the Picture to Zoom In
-
ARG10759 anti-mCherry antibody [1C51] ICC/IF image
Immunocytochemistry: HEK293 cells transfected with mCherry and visualized in red. The cells were stained with ARG10759 anti-mCherry antibody [1C51] in green, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mCherry and the ARG10759 anti-mCherry antibody [1C51]. Untransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue).
-
ARG10759 anti-mCherry antibody [1C51] WB image
Western blot: Crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector in lane labelled "+". The "-" lane is a blot of an equal amount of protein extract from untransfected HEK293 cells. ARG10759 anti-mCherry antibody [1C51] binds a major band running at ~28 kDa corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected HEK293 cells.
-
ARG10759 anti-mCherry antibody [1C51] WB image
Western blot: 1) HEK293 (non-transfected), 2) HEK293 transfected with mCherry-HA construct, 3) Recombinant mCherry protein, 4) Recombinant GFP protein and 5) HEK293 transfected with GFP construct. The blots were stained with ARG10759 anti-mCherry antibody [1C51] (green) at 1:1000 dilution. The same blot was simultaneously stained with ARG10757 anti-Hsp 60 antibody (red) at 1:5000 dilution.
