ARG55723
anti-mCherry antibody
anti-mCherry antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,IHC-Whole mount,Western blot and Other
Publication6
Overview
| Product Description | Goat Polyclonal antibody recognizes mCherry |
|---|---|
| Tested Reactivity | Other |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, IHC-Wmt, WB |
| Specificity | This antibody recognizes mCherry, red fluorescent protein (dsRed) and tdTomato. It does not cross-react to GFP. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | mCherry |
| Immunogen | Purified recombinant peptide produced in E. coli |
| Conjugation | Un-conjugated |
| Alternate Names | Cherry fluorescent protein; dsRed; red fluorescent protein; tdTomato |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.05% Sodium azide and 20% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 20% Glycerol |
| Concentration | 3 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | mCherry protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. |
|---|---|
| Highlight | Related news: tdTomato, mRuby, and mCherry antibodies for triple staining; |
Images (2) Click the Picture to Zoom In
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ARG55723 anti-mCherry antibody WB image
Western blot: 100 µg of 293HEK cells transduced with myc-mCherry Ad and stained with ARG55723 anti-mCherry antibody at 1:1000 dilution.
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ARG55723 anti-mCherry antibody ICC/IF image
Immunocytochemistry: 293HEK cells transfected with mCherry-Rab1a and stained with ARG55723 anti-mCherry antibody at 1:50 dilution. Cells were fixed with 4% of PFA.
Specific References
Her4.3+ radial glial cells maintain the brain vascular network through activation of Wnt signaling
IHC-Wmt / Zefrafish
Inhibition of HDAC activity directly reprograms murine embryonic stem cells to trophoblast stem cells
ICC/IF / Human
The MRN complex maintains the biliary-derived hepatocytes in liver regeneration through ATR-Chk1 pathway
IHC-Wmt / Zefrafish
VEGF signaling governs the initiation of biliary-mediated liver regeneration through the PI3K-mTORC1 axis
IHC-Wmt / Zebrafish
Extensive jejunal injury is repaired by migration and transdifferentiation of ileal enterocytes in zebrafish
IHC-Wmt / Zefrafish
Tel2 regulates redifferentiation of bipotential progenitor cells via Hhex during zebrafish liver regeneration
IHC / Zefrafish
