ARG82862

arigoPLEX® Human Proinflammatory Cytokine Multiplex ELISA Kit (IFN gamma, IL1 beta, IL6 and TNF alpha)

Datasheet

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96 wells

739.00

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Component

Cat No	Component Name	Package	Temp
ARG82862-01	Antibody Coated Microplate	8 X 12 strips	4°C
ARG82862-02	Standards Mixture	3 vials	4°C
ARG82862-03	100X Antibody Conjugate Mixture	120 μl	≤ -20°C
ARG82862-04	100X HRP-Streptavidin Solution	120 μl	4°C
ARG82862-05	Standard / Sample Diluent Buffer	30 ml	4°C
ARG82862-06	Antibody Diluent Buffer	35 ml	4°C
ARG82862-07	20X Wash Buffer	45 ml	4°C
ARG82862-08	TMB substrate	12 ml	4°C (protect from light)
ARG82862-09	STOP solution	12 ml	4°C
ARG82862-10	Plate sealer	3 adhesive strips	Room temperature

Overview

Product Description	ARG82862 arigoPLEX® Human Proinflammatory Cytokine Multiplex ELISA Kit (IFN gamma, IL1 beta, IL6 and TNF alpha) is an Enzyme Immunoassay kit for the quantification of Human Proinflammatory Cytokine (IFN gamma, IL1 beta, IL6 and TNF alpha) in serum, plasma and cell culture supernatants. See all Multiplex ELISA kits
Tested Reactivity	Hu
Tested Application	ELISA
Target Name	ProInflammatory Cytokine
Conjugation	HRP
Conjugation Note	Substrate: TMB and read at 450 nm.
Sensitivity	IFN gamma: 7.8 pg/ml IL1 beta: 7.8 pg/ml IL6: 15.6 pg/ml TNF alpha: 15.6 pg/ml
Sample Type	Serum, plasma and cell culture supernatants.
Standard Range	IFN gamma: 15.6 - 500 pg/ml IL1 beta: 15.6 - 500 pg/ml IL6: 31.25 - 1000 pg/ml TNF alpha: 31.25 - 1000 pg/ml
Sample Volume	50 µl

Application Instructions

Assay Time	4.5 hours

Properties

Form	96 well
Storage Instruction	Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol	IFNG; IL1B; IL6; TNF
Gene Full Name	Interferon Gamma; Interleukin 1 Beta; Interleukin 6 ; Tumor Necrosis Factor
Background	IFN gamma:This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015] IL1 beta:The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2020] IL6:This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). [provided by RefSeq, Aug 2020] TNF alpha:This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020]
Function	IFN gamma:Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation. [UniProt] IL1 beta:Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection. [UniProt] IL6:Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity). [UniProt] TNF alpha:Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6. Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity). [UniProt]
Highlight	Related Product: IFN gamma antibodies; IL1-beta antibodies; IL6 antibodies; TNF alpha antibodies; Related news: Inflammatory Cytokine Multiplex ELISA Kits are launched;

Images (4) Click the Picture to Zoom In

ARG82862 arigoPLEX® Human Proinflammatory Cytokine Multiplex ELISA Kit (IFN gamma, IL1 beta, IL6, TNF alpha) standard curve image

ARG82862 arigoPLEX^® Human Proinflammatory Cytokine Multiplex ELISA Kit (IFN gamma, IL1 beta, IL6, TNF alpha) results of a typical standard for Human IFN gamma run with optical density reading at 450 nm.
ARG82862 arigoPLEX® Human Proinflammatory Cytokine Multiplex ELISA Kit (IFN gamma, IL1 beta, IL6, TNF alpha) standard curve image

ARG82862 arigoPLEX® Human Proinflammatory Cytokine Multiplex ELISA Kit (IFN gamma, IL1 beta, IL6, TNF alpha) results of a typical standard for Human IL1 beta run with optical density reading at 450 nm.
ARG82862 arigoPLEX® Human Proinflammatory Cytokine Multiplex ELISA Kit (IFN gamma, IL1 beta, IL6, TNF alpha) standard curve image

ARG82862 arigoPLEX^® Human Proinflammatory Cytokine Multiplex ELISA Kit (IFN gamma, IL1 beta, IL6, TNF alpha) results of a typical standard for Human IL6 run with optical density reading at 450 nm.
ARG82862 arigoPLEX® Human Proinflammatory Cytokine Multiplex ELISA Kit (IFN gamma, IL1 beta, IL6, TNF alpha) standard curve image

ARG82862 arigoPLEX^® Human Proinflammatory Cytokine Multiplex ELISA Kit (IFN gamma, IL1 beta, IL6, TNF alpha) results of a typical standard for Human TNF alpha run with optical density reading at 450 nm.