ARG82127

Acetylcholinesterase Activity Assay Kit (Colorimetric)

Acetylcholinesterase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All species

Overview

Product Description ARG82127 Acetylcholinesterase Activity Assay Kit (Colorimetric) can be used to measure Acetylcholinesterase activity in serum, plasma and blood.
Tested Reactivity Hu, Ms, Rat, All
Tested Application FuncSt
Target Name Acetylcholinesterase
Conjugation Note Read at 412 nm
Sensitivity 10 U/L
Detection Range 10 - 600 U/L
Sample Type serum, plasma and blood
Sample Volume 10 µl
Alternate Names ARACHE; Acetylcholinesterase; ACEE; EC 3.1.1.7; AChE; N-ACHE; YT

Application Instructions

Application Note Please note that this kit does not include a microplate.
Assay Time 10 min

Properties

Form Liquid
Storage Instruction Store the kit at 2-8°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11423 Mouse ACHE

GeneID: 43 Human ACHE

Swiss-port # P21836 Mouse Acetylcholinesterase

Swiss-port # P22303 Human Acetylcholinesterase

Gene Symbol ACHE
Gene Full Name acetylcholinesterase (Yt blood group)
Background Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]
Function Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. [UniProt]
Cellular Localization Cell junction, synapse. Secreted. Cell membrane; Peripheral membrane protein. Isoform T: Nucleus. Note=Only observed in apoptotic nuclei. Isoform H: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. [UniProt]
Title Download Link
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