ARG82644

Canine IL1 alpha ELISA Kit

Canine IL1 alpha ELISA Kit for ELISA and Dog

Overview

Product Description ARG82644 Canine IL1 alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Canine IL1 alpha in serum, plasma, cell lysate and tissue lysate.
Tested Reactivity Dog
Tested Application ELISA
Specificity The following recombinant canine proteins were tested and exhibited no cross-reactivity or interference: ApoA1, BMP1, BMP2, BMP3, BMP4, CCL4 / MIP1 beta, CRP, HSP27, IL1 beta, IL1RA, IL2, IL4, IL5, IL8, IL10, IL12, IL15, IL17C, IL21, IL23, IL2R, IFN gamma, PDGF, PLA2G7, prolactin, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3, TNF alpha, TNF RI, TNF RII and VEGF.
Target Name IL1 alpha
Conjugation HRP
Conjugation Note Read at 450 nm.
Sensitivity 1.3 pg/ml
Sample Type Serum, plasma, cell lysate and tissue lysate.
Standard Range 2.5 - 160 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6%
Inter-Assay CV: 9%
Alternate Names IL-1 alpha; Interleukin-1 alpha; IL1; IL1F1; Hematopoietin-1; IL1-ALPHA; IL-1A

Application Instructions

Assay Time ~ 3 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL1A
Gene Full Name interleukin 1, alpha
Background The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]
Function Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
Cellular Localization Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. [UniProt]
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Title Download Link
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