ARG82125

Corticotropin Releasing Factor ELISA Kit

Corticotropin Releasing Factor ELISA Kit for ELISA and Cat,Dog,Human,Mouse,Rat

Overview

Product Description ARG82125 Corticotropin Releasing Factor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Corticotropin Releasing Factor in serum and plasma.
Tested Reactivity Hu, Ms, Rat, Cat, Dog
Species Does Not React With Bov, Sheep
Tested Application ELISA
Specificity Cross Reactivity:
CRF (Human, Rat): 100%
CRF (Sheep, Bovine), Prepro-CRF (125-151, Human), PACAP-38 (Human, Rat, Sheep), LH-RH, ACTH (Human), [Arg8]-Vasopressin and BNP-45 (Rat): 0%
Target Name Corticotropin Releasing Factor
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.33 ng/ml
Detection Range 0.33 - 3.73 ng/ml
Sample Type Serum and plasma.
Standard Range 0.01 - 100 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: < 10%
Inter-Assay CV: < 15%
Alternate Names Corticoliberin; Corticotropin-releasing hormone; CRF; CRH1; Corticotropin-releasing factor

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12918 Mouse CRH

GeneID: 1392 Human CRH

GeneID: 81648 Rat CRH

Gene Symbol CRH
Gene Full Name corticotropin releasing hormone
Background Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Apr 2010]
Function This hormone from hypothalamus regulates the release of corticotropin from pituitary gland. [UniProt]
Cellular Localization Secreted. [UniProt]
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Title Download Link
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