ARG70358
Dengue virus NS1 recombinant protein (His-tagged, C-ter)
Dengue virus NS1 recombinant protein (His-tagged, C-ter) for SDS-PAGE and Virus
Microbiology and Infectious Disease antibody
Overview
| Product Description | 293T cell expressed, His-tagged (C-ter) Dengue virus NS1 recombinant protein. |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | SDS-PAGE |
| Target Name | Dengue virus NS1 protein |
| Species | Virus |
| A.A. Sequence | MDSGC VVSWK NKELK CGSGI FITDN VHTWT EQYK FQPES PSKLA SAIQK AHEEG ICGIR SVTRL ENLMW KQITP ELNHI LSENE VKLTI MTGDI KGIMQ AGKRS LRPQP TELKY SWKTW GKAKM LSTES HNQTF LIDGP ETAEC PNTNR AWNSL EVEDY GFGVF TTNIW LKLKE KQDVF CDSKL MSAAI KDNRA VHADM GYWIE SALND TWKIE KASFI EVKNC HWPKS HTLWS NGVLE SEMII PKNLA GPVSQ HNYRP GYHTQ ITGPW HLGKL EMDFD FCDGT TVVVT EDCGN RGPSL RTTTA SGKLI TEWCC RSCTL PPLRY RGEDG CWYGM EIRPL KEKEE NLVNS LVTA with polyhistidine tag at the C-terminus. |
| Expression System | 293T cell |
| Protein Full Name | Dengue virus nonstructural protein 1 |
| Alternate Names | Dengue virus NS1 protein; Dengue virus nonstructural glycoprotein NS1 protein |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. DO NOT vortex. If a further dilution is needed, dilute reconstituted protein with carrier protein (0.1% BSA or 5% HSA or 10% FBS) containing PBS or medium is recommended. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | DENV_gp1 |
|---|---|
| Gene Full Name | Dengue virus nonstructural protein 1 |
| Background | Dengue virus NS1 protein is a nonstructural protein which could be secreted and have been developed as diagnostic biomarker for early detection. There are several forms of NS1 including monomer, dimer, and hexamer during infection. Dimeric NS1 can be anchored to cell membranes with glycosyl-phosphatidylinositol (GPI). Hexameric NS1 can be secreted and detected in patients' blood samples (up to 50 μg/mL) or infected cell supernatants (various from ng/mL to μg/mL depend on serotypes and strains). Studies have shown that NS1 could interfere complement activity and prothrombin activation. In addition, NS1 could elicit antibodies which cross-react with host antigens including coagulation factors and molecules expressed in endothelial cells and platelets through molecular mimic. |
| Function | Dengue virus (DENV) non-structural protein 1 (NS1) is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome. [Uniprot] |
| Highlight | Related Antibody Duos and Panels: ARG30252 Dengue virus NS1 ELISA Antibody Duo Related products: Dengue virus antibodies; Dengue virus ELISA Kits; Dengue virus Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Best NS1 antibodies in the market Fighting fire with fire: Genetically-engineered mosquitoes as an alternative to fight diseases Exploring Antiviral Immune Response |
| Research Area | Microbiology and Infectious Disease antibody |
