ARG70123
FGF17 recombinant protein (Active) (His-tagged, N-ter)
FGF17 recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE and Human,Mouse
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human FGF17 recombinant protein |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | SDS-PAGE |
| Target Name | FGF17 |
| Species | Human |
| A.A. Sequence | Human FGF17 sequence: TQGEN HPSPN FNQYV RDQGA MTDQL SRRQI REYQL YSRTS GKHVQ VTGRR ISATA EDGNK FAKLI VETDT FGSRV RIKGA ESEKY ICMNK RGKLI GKPSG KSKDC VFTEI VLENN YTAFQ NARHE GWFMA FTRQG RPRQA SRSRQ NQREA HFIKR LYQGQ LPFPN HAEKQ KQFEF VGSAP TRRTK RTRRP QPLT with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 5 ng/ml. The specific activity of recombinant human FGF-17 is > 2 x 10^5 IU/mg |
| Alternate Names | FGF-17; HH20; FGF-13; Fibroblast growth factor 17 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FGF17 |
| Gene Full Name | fibroblast growth factor 17 |
| Background | This gene encodes a member of the fibroblast growth factor (FGF) family. Member of the FGF family possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is expressed during embryogenesis and in the adult cerebellum and cortex and may be essential for vascular growth and normal brain development. Mutations in this gene are the cause of hypogonadotropic hypogonadism 20 with or without anosmia. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
| Function | Plays an important role in the regulation of embryonic development and as signaling molecule in the induction and patterning of the embryonic brain. Required for normal brain development. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
