ARG70114
FGF8 (isoform A) recombinant protein (Active) (His-tagged, C-ter)
FGF8 (isoform A) recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE and Human,Mouse
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FGF8 (isoform A) recombinant protein |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | SDS-PAGE |
| Target Name | FGF8 (isoform A) |
| Species | Human |
| A.A. Sequence | Human FGF8 sequence: MQHVR EQSLV TDQLS RRLIR TYQLY SRTSG KHVQV LANKR INAMA EDGDP FAKLI VETDT FGSRV RVRGA ETGLY ICMNK KGKLI AKSNG KGKDC VFTEI VLENN YTALQ NAKYE GWYMA FTRKG RPRKG SKTRQ HQREV HFMKR LPRGH HTTEQ SLRFE FLNYP PFTRS LRGSQ RTWAP EPR with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 2 μg/ml. |
| Alternate Names | Fibroblast growth factor 8; HH6; AIGF; FGF-8; HBGF-8; KAL6; Heparin-binding growth factor 8; Androgen-induced growth factor |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FGF8 |
| Gene Full Name | fibroblast growth factor 8 (androgen-induced) |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008] |
| Function | Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Plays a role in neurite outgrowth in hippocampal cells. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
