ARG82165

Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric)

Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All species

Overview

Product Description ARG82165 Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric) can be used to measure Glucose 6 phosphate Dehydrogenase activity in serum, plasma, tissue and cell culture supernatants.
Tested Reactivity Hu, Ms, Rat, All
Tested Application FuncSt
Target Name Glucose 6 phosphate Dehydrogenase
Conjugation Note Read at 460 nm
Sensitivity 0.2 U/L
Detection Range 0.2 - 100 U/L
Sample Type serum, plasma, tissue and cell culture supernatants
Sample Volume 20 µl
Alternate Names G6PD1; G6PD; EC 1.1.1.49; Glucose-6-phosphate 1-dehydrogenase

Application Instructions

Application Note Please note that this kit does not include a microplate.
Assay Time 20 min

Properties

Form Liquid
Storage Instruction Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24377 Rat G6PD

GeneID: 2539 Human G6PD

Swiss-port # P05370 Rat Glucose-6-phosphate 1-dehydrogenase

Swiss-port # P11413 Human Glucose-6-phosphate 1-dehydrogenase

Gene Symbol G6PD
Gene Full Name glucose-6-phosphate dehydrogenase
Background This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. [UniProt]
PTM Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity. [UniProt]
Title Download Link
ARG82165 Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric) User's manual download icon Download