ARG82767
Glutaminase Assay Kit (Colorimetric)
Glutaminase Assay Kit (Colorimetric) for Functional study and All species
Overview
| Product Description | ARG82767 Glutaminase Assay Kit (Colorimetric) is a detection kit for the quantification of Glutaminase activity in cells, bacteria and tissue samples. |
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| Tested Reactivity | All |
| Tested Application | FuncSt |
| Specificity | The assay is initiated with the enzymatic hydrolysis of Glutamine by Glutaminase. The enzyme catalysed reaction products can be measured at a colorimetric readout at 420 nm. |
| Target Name | Glutaminase |
| Conjugation Note | Read at 420 nm (colorimetric) |
| Detection Range | 0.01 - 1 µmol/ml |
| Sample Type | Cells, bacteria and tissue samples. |
| Sample Volume | 20 µl |
| Alternate Names | EC 3.5.1.2; AAD20; L-glutamine amidohydrolase; KGA; Glutaminase kidney isoform, mitochondrial; GAM; K-glutaminase; GLS; GAC; GLS1 |
Application Instructions
| Assay Time | ~ 1.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | GLS |
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| Gene Full Name | glutaminase |
| Background | This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
| Function | Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed:30575854, PubMed:30239721, PubMed:30970188). [Isoform 2]: Lacks catalytic activity. [UniProt] |
| Cellular Localization | Isoform 1: Cytoplasm, cytosol. Isoform 3: Mitochondrion. [UniProt] |
| Highlight | Related products: Glutaminase antibodies; Glutaminase ELISA Kits; Related news: TCA intermediate fumarate promotes mitobiogenesis |
| Title | Download Link |
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| ARG82767 Glutaminase Assay Kit (Colorimetric) User's manual |
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