ARG70223
HCoV-229E nucleocapsid recombinant protein (His-tagged, C-ter)
HCoV-229E nucleocapsid recombinant protein (His-tagged, C-ter) for SDS-PAGE and Virus
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) HCoV-229E nucleocapsid recombinant protein |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | SDS-PAGE |
| Target Name | HCoV-229E nucleocapsid protein |
| Species | Virus |
| A.A. Sequence | MATVK WADAS EPQRG RQGRI PYSLY SPLLV DSEQP WKVIP RNLVP INKKD KNKLI GYWNV QKRFR TRKGK RVDLS PKLHF YYLGT GPHKD AKFRE RVEGV VWVAV DGAKT EPTGY GVRRK NSEPE IPHFN QKLPN GVTVV EEPDS RAPSR SQSRS QSRGR GESKP QSRNP SSDRN HNSQD DIMKA VAAAL KSLGF DKPQE KDKKS AKTGT PKPSR NQSPA SSQTS AKSLA RSQSS ETKEQ KHEMQ KPRWK RQPND DVTSN VTQCF GPRDL DHNFG SAGVV ANGVK AKGYP QFAEL VPSTA AMLFD SHIVS KESGN TVVLT FTTRV TVPKD HPHLG KFLEE LNAFT REMQQ HPLLN PSALE FNPSQ TSPAT AEPVR DEVSI ETDII DEVN with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
