ARG70222

HCoV-HKU1 nucleocapsid recombinant protein (His-tagged, C-ter)

HCoV-HKU1 nucleocapsid recombinant protein (His-tagged, C-ter) for SDS-PAGE and Virus

Overview

Product Description E. coli expressed, His-tagged (C-ter) HCoV-HKU1 nucleocapsid recombinant protein
Tested Reactivity Virus
Tested Application SDS-PAGE
Target Name HCoV-HKU1 nucleocapsid protein
Species Virus
A.A. Sequence MSYTP GHYAG SRSSS GNRSG ILKKT SWADQ SERNY QTFNR GRKTQ PKFTV STQPQ GNTIP HYSWF SGITQ FQKGR DFKFS DGQGV PIAFG VPPSE AKGYW YRHSR RSFKT ADGQQ KQLLP RWYFY YLGTG PYANA SYGES LEGVF WVANH QADTS TPSDV SSRDP TTQEA IPTRF PPGTI LPQGY YVEGS GRSAS NSRPG SRSQS RGPNN RSLSR SNSNF RHSDS IVKPD MADEI ANLVL AKLGK DSKPQ QVTKQ NAKEI RHKIL TKPRQ KRTPN KHCNV QQCFG KRGPS QNFGN AEMLK LGTND PQFPI LAELA PTPGA FFFGS KLDLV KRDSE ADSPV KDVFE LHYSG SIRFD STLPG FETIM KVLEE NLNAY VNSNQ NTDSD SLSSK PQRKR GVKQL PEQFD SLNLS AGTQH ISNDF TPEDH SLLAT LDDPY VEDSV A with polyhistidine tag at the C-terminus.
Expression System E. coli

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.