ARG70213
HCoV-NL63 nucleocapsid recombinant protein (His-SUMO tagged, N-ter)
HCoV-NL63 nucleocapsid recombinant protein (His-SUMO tagged, N-ter) for SDS-PAGE and Virus
Overview
| Product Description | HEK293 expressed, His-SUMO tagged (N-ter) HCoV-NL63 nucleocapsid recombinant protein. |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | SDS-PAGE |
| Target Name | HCoV-NL63 nucleocapsid protein |
| Species | Virus |
| A.A. Sequence | MASVN WADDR AARKK FPPPS FYMPL LVSSD KAPYR VIPRN LVPIG KGNKD EQIGY WNVQE RWRMR RGQRV DLPPK VHFYY LGTGP HKDLK FRQRS DGVVW VAKEG AKTVN TSLGN RKRNQ KPLEP KFSIA LPPEL SVVEF EDRSN NSSRA SSRSS TRNNS RDSSR STSRQ QSRTR SDSNQ SSSDL VAAVT LALKN LGFDN QSKSP SSSGT STPKK PNKPL SQPRA DKPSQ LKKPR WKRVP TREEN VIQCF GPRDF NHNMG DSDLV QNGVD AKGFP QLAEL IPNQA ALFFD SEVST DEVGD NVQIT YTYKM LVAKD NKNLP KFIEQ ISAFT KPSSI KEMQS QSSHV AQNTV LNASI PESKP LADDD SAIIE IVNEV LH with polyhistidine-SUMO tag at the N-terminus. |
| Expression System | HEK293 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
