ARG70221

HCoV-OC43 nucleocapsid recombinant protein (His-tagged, C-ter)

HCoV-OC43 nucleocapsid recombinant protein (His-tagged, C-ter) for SDS-PAGE and Virus

Overview

Product Description E. coli expressed, His-tagged (C-ter) HCoV-OC43 nucleocapsid recombinant protein
Tested Reactivity Virus
Tested Application SDS-PAGE
Target Name HCoV-OC43 nucleocapsid protein
Species Virus
A.A. Sequence MSFTP GKQSS SRASS GNRSG NGILK WADQS DQVRN VQTRG RRAQP KQTAT SQQPS GGNVV PYYSW FSGIT QFQKG KEFEF VEGQG PPIAP GVPAT EAKGY WYRHN RGSFK TADGN QRQLL PRWYF YYLGT GPHAK DQYGT DIDGV YWVAS NQADV NTPAD IVDRD PSSDE AIPTR FPPGT VLPQG YYIEG SGRSA PNSRS TSRTS SRASS AGSRS RANSG NRTPT SGVTP DMADQ IASLV LAKLG KDATK PQQVT KHTAK EVRQK ILNKP RQKRS PNKQC TVQQC FGKRG PNQNF GGGEM LKLGT SDPQF PILAE LAPTA GAFFF GSRLE LAKVQ NLSGN PDEPQ KDVYE LRYNG AIRFD STLSG FETIM KVLNE NLNAY QQQDG MMNMS PKPQR QRGHK NGQGE NDNIS VAVPK SRVQQ NKSRE LTAED ISLLK KMDEP YTEDT SEI with polyhistidine tag at the C-terminus.
Expression System E. coli

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.