ARG82791

Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit

Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit for ELISA and Human

Metabolism kit; Neuroscience kit; Signaling Transduction kit

Overview

Product Description ARG82791 Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit is an Enzyme Immunoassay kit for the quantification of 2-MET (Metanephrine + Normetanephrine) in Human plasma (EDTA, heparin).
Tested Reactivity Hu
Tested Application ELISA
Specificity Cross-Reactivity (Metanephrine):
Derivatized Metanephrine: 100%
Derivatized Normetanephrine: 0.05%
3-Methoxytyramin, Adrenaline, Noradrenaline, Dopamin, Vanillic mandelic acid, Homovanillic acid, L-DOPA, L-Tyrosin, Tyramine, Normetanephrine, Acetaminophen: < 0.01%

Cross-Reactivity (Normetanephrine):
Derivatized Metanephrine: 0.72%
Derivatized Normetanephrine: 100%
3-Methoxytyramin: 6.5%(*)
Adrenaline, Noradrenaline, Dopamin, Vanillic mandelic acid, Homovanillic acid, L-DOPA, L-Tyrosin, Tyramine, Normetanephrine, Acetaminophen: < 0.01%

* Note: Normetanephrine concentrations are not influenced by 3-Methoxytyramine in case of normal 3-Methoxytyramine concentrations. Only very high 3-Methoxytyramine concentrations found in rare cases of exclusively dopamine secreting tumors can cause false positive results.
Target Name 2-MET (Metanephrine + Normetanephrine)
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity Metanephrine: 14.9 pg/ml
Normetanephrine: 17.9 pg/ml
Sample Type Plasma (EDTA, heparin).
Standard Range Metanephrine: 36 - 3600 pg/ml
Normetanephrine: 72 - 7200 pg/ml
Sample Volume 200 µl
Precision Metanephrine: Intra-Assay CV: 11.2% ; Inter-Assay CV: 12.2%
Normetanephrine: Intra-Assay CV: 9.3% ; Inter-Assay CV: 6.7%

Application Instructions

Assay Time 5 hours 25 min

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

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Research Area Metabolism kit; Neuroscience kit; Signaling Transduction kit
Title Download Link
ARG82791 Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit User's manual download icon Download