ARG82791
Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit
Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit for ELISA and Human
Metabolism kit; Neuroscience kit; Signaling Transduction kit
Overview
Product Description | ARG82791 Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit is an Enzyme Immunoassay kit for the quantification of 2-MET (Metanephrine + Normetanephrine) in Human plasma (EDTA, heparin). |
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Tested Reactivity | Hu |
Tested Application | ELISA |
Specificity | Cross-Reactivity (Metanephrine): Derivatized Metanephrine: 100% Derivatized Normetanephrine: 0.05% 3-Methoxytyramin, Adrenaline, Noradrenaline, Dopamin, Vanillic mandelic acid, Homovanillic acid, L-DOPA, L-Tyrosin, Tyramine, Normetanephrine, Acetaminophen: < 0.01% Cross-Reactivity (Normetanephrine): Derivatized Metanephrine: 0.72% Derivatized Normetanephrine: 100% 3-Methoxytyramin: 6.5%(*) Adrenaline, Noradrenaline, Dopamin, Vanillic mandelic acid, Homovanillic acid, L-DOPA, L-Tyrosin, Tyramine, Normetanephrine, Acetaminophen: < 0.01% * Note: Normetanephrine concentrations are not influenced by 3-Methoxytyramine in case of normal 3-Methoxytyramine concentrations. Only very high 3-Methoxytyramine concentrations found in rare cases of exclusively dopamine secreting tumors can cause false positive results. |
Target Name | 2-MET (Metanephrine + Normetanephrine) |
Conjugation | HRP |
Conjugation Note | Substrate: TMB and read at 450 nm. |
Sensitivity | Metanephrine: 14.9 pg/ml Normetanephrine: 17.9 pg/ml |
Sample Type | Plasma (EDTA, heparin). |
Standard Range | Metanephrine: 36 - 3600 pg/ml Normetanephrine: 72 - 7200 pg/ml |
Sample Volume | 200 µl |
Precision | Metanephrine: Intra-Assay CV: 11.2% ; Inter-Assay CV: 12.2% Normetanephrine: Intra-Assay CV: 9.3% ; Inter-Assay CV: 6.7% |
Application Instructions
Assay Time | 5 hours 25 min |
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Properties
Form | 96 well |
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Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Highlight | New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
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Research Area | Metabolism kit; Neuroscience kit; Signaling Transduction kit |
Title | Download Link |
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ARG82791 Human 2-MET (Metanephrine + Normetanephrine) ELISA Kit User's manual | Download |