ARG80351
Human ACPA ELISA Kit
Human ACPA ELISA Kit for ELISA and Human
Signaling Transduction kit
Publication1
Overview
| Product Description | ARG80351 Human ACPA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ACPA (Anti-citrullinated protein antibodies) in serum and plasma. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | ACPA |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 1 U/ml |
| Sample Type | Serum and plasma. |
| Standard Range | 20 - 1000 U/ml |
| Sample Volume | 10 µl |
| Alternate Names | ACPA; Anti-citrullinated protein antibodies; anti-cyclic citrullinated peptides; cyclic citrullinated peptides antibody; CCP antibody; cyclic citrullinated peptides autoantibody; CCP autoantibody; |
Application Instructions
| Assay Time | 30, 15, 15, 5 min (RT) |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | Rheumatoid arthritis (RA) is one of the most common autoimmune diseases. The main characteristic of RA is joint inflammation that results in joint damage and loss of function. An early diagnosis of RA and an immediate beginning of an appropriate treatment is important to prevent a complete joint damage. RA is diagnosed primarily on clinical manifestations and serological support has, up to now, been mainly restricted to the determination of autoantibodies against rheumatoid factor (RF). RF is a sensitive serological marker for RA with a moderate specificity of about 70%. In several studies, it has been demonstrated that the determination of antibodies against citrullinated arginine residues in filament proteins occurs in RF-negative patients. Citrullination is a peptidyl arginine deiminase (PAD) catalyzed process in which the amino acid arginine (Arg) is modified to citrulline. During this conversion, the positively charged NH2-group is hydrolyzed to an oxygen group. ACPA ELISA shows both a high specificity and a high sensitivity for auto-antibodies against citrullinated vimentin. Vimentin is an omnipresent citrullinated protein which was observed in the rheumatoid synovial tissue of RA patients. There are recent findings of secernation and modification of vimentin by macrophages depending on pro-inflammatory signals. The titer of antibodies against vimentin in RA patients strongly correlates with the disease activity score (DAS). |
| Highlight | Related products: ACPA ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Signaling Transduction kit |
Specific References
ITGA2 protein is associated with rheumatoid arthritis in Chinese and affects cellular function of T cells
ELISA / Human / Plasma
| Title | Download Link |
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| ARG80351 Human ACPA ELISA Kit User's manual |
 Download
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