ARG81485

Human ADAM12 ELISA Kit

Human ADAM12 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81485-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81485-002 Standard 2 X 10 ng/vial 4°C
ARG81485-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81485-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81485-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81485-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81485-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81485-008 25X Wash buffer 20 ml 4°C
ARG81485-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81485-010 STOP solution 10 ml (Ready to use) 4°C
ARG81485-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81485 Human ADAM12 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ADAM12 in serum, plasma (heparin), urine and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name ADAM12
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin), urine and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.5%
Inter-Assay CV: 7.1%
Alternate Names MCMP; ADAM 12; MCMPMltna; EC 3.4.24.-; Disintegrin and metalloproteinase domain-containing protein 12; Meltrin-alpha; MLTN; MLTNA; CAR10; ADAM12-OT1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8038 Human ADAM12

Swiss-port # O43184 Human Disintegrin and metalloproteinase domain-containing protein 12

Gene Symbol ADAM12
Gene Full Name ADAM metallopeptidase domain 12
Background This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]
Function Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity). [UniProt]
Highlight Related products:
ADAM antibodies; ADAM ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM The precursor is cleaved by a furin endopeptidase. [UniProt]
Title Download Link
ARG81485 Human ADAM12 ELISA Kit User manual download icon Download