ARG81483

Human ADAMTS13 ELISA Kit

Human ADAMTS13 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81483-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81483-002 Standard 2 X 50 ng/vial 4°C
ARG81483-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81483-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81483-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81483-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81483-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81483-008 25X Wash buffer 20 ml 4°C
ARG81483-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81483-010 STOP solution 10 ml (Ready to use) 4°C
ARG81483-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81483 Human ADAMTS13 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ADAMTS13 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name ADAMTS13
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.7%
Inter-Assay CV: 6.1%
Alternate Names von Willebrand factor-cleaving protease; vWF-CP; EC 3.4.24.87; ADAM-TS 13; VWFCP; A disintegrin and metalloproteinase with thrombospondin motifs 13; ADAM-TS13; vWF-cleaving protease; ADAMTS-13; C9orf8

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11093 Human ADAMTS13

Swiss-port # Q76LX8 Human A disintegrin and metalloproteinase with thrombospondin motifs 13

Gene Symbol ADAMTS13
Gene Full Name ADAM metallopeptidase with thrombospondin type 1 motif, 13
Background This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Function Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. [UniProt]
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ADAMTS antibodies; ADAMTS ELISA Kits;
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PTM Glycosylated. O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS13. May also be C-glycosylated on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and also N-glycosylated. These other glycosylations can also facilitate secretion.

The precursor is processed by a furin endopeptidase which cleaves off the pro-domain. [UniProt]
Title Download Link
ARG81483 Human ADAMTS13 ELISA Kit User's manual download icon Download