ARG81565

Human ADAMTS4 ELISA Kit

Human ADAMTS4 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81565-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81565-002 Standard 2 X 40 ng/vial 4°C
ARG81565-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81565-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81565-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81565-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81565-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81565-008 25X Wash buffer 20 ml 4°C
ARG81565-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81565-010 STOP solution 10 ml (Ready to use) 4°C
ARG81565-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81565 Human ADAMTS4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ADAMTS4 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name ADAMTS4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 312.5 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 625 - 40000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.7%
Inter-Assay CV: 5.8%
Alternate Names ADAMTS-4; Aggrecanase-1; ADAMTS-2; EC 3.4.24.82; A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADMP-1; ADAM-TS 4

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9507 Human ADAMTS4

Swiss-port # O75173 Human A disintegrin and metalloproteinase with thrombospondin motifs 4

Gene Symbol ADAMTS4
Gene Full Name ADAM metallopeptidase with thrombospondin type 1 motif, 4
Background This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq, Jul 2008]
Function Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site. [UniProt]
Highlight Related products:
ADAMTS antibodies; ADAMTS ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM The precursor is cleaved by a furin endopeptidase.

Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity). [UniProt]
Title Download Link
ARG81565 Human ADAMTS4 ELISA Kit User's manual download icon Download