ARG81635
Human AREG / Amphiregulin ELISA Kit
Human AREG / Amphiregulin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81635-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81635-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81635-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81635-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81635-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81635-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81635-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81635-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81635-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81635-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81635-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81635 Human AREG / Amphiregulin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human AREG / Amphiregulin in serum, plasma (heparin, EDTA), saliva, human milk and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | AREG / Amphiregulin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva, human milk and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9%; Inter-Assay CV: 6.7% |
| Alternate Names | Amphiregulin; AREGB; CRDGF; SDGF; AR; Colorectum cell-derived growth factor |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AREG |
| Gene Full Name | amphiregulin |
| Background | The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014] |
| Function | Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. [UniProt] |
| Highlight | Related products: AREG antibodies; AREG ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG81635 Human AREG / Amphiregulin ELISA Kit User's manual |
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