ARG80834

Human Androstenedione ELISA Kit

Human Androstenedione ELISA Kit for ELISA and Human

Signaling Transduction kit

Overview

Product Description ARG80834 Human Androstenedione ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Androstenedione in serum and plasma (EDTA).
Tested Reactivity Hu
Tested Application ELISA
Target Name Androstenedione
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.021 ng/ml
Sample Type Serum and plasma (EDTA).
Standard Range 0.1 - 10 ng/ml
Sample Volume 20 µl

Application Instructions

Assay Time 1 h, 30 min (RT)

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Androstenedione
Background The steroid hormone Androstenedione is one of the main androgens, besides Testosterone and Dehydroepiandrosterone. Testosterone, the most important biological active androgen, is derived from peripheral enzymatic conversion of Androstenedione. In males, androgens are secreted primarily by the Leydig cells of the testes, to some degree also in the adrenal cortex. In females, the androgens are secreted mainly in the adrenal glands and in the ovary.

Around 10% of the androgens are derived from peripheral conversion, mainly of DHEA. Androstenedione and Testosterone show high diurnal variability. The highest levels are measured in the morning. At the age of puberty serum androstenedione levels rise, after menopause they decline again. High androstenedione levels are measured during pregnancy.

In women, high levels of androstenedione (47-100% above normal) are generally found in hirsutism, mostly in combination with other androgens as testosterone and DHEA-S. Androstenedione overproduction is due to ovarian dysfunction or may be of adrenal origin. High circulating androstenedione levels are found in women with polycystic ovaries and 21-hydroxylase effect. Significant lower androstenedione levels are found in postmenopausal osteoporosis.
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Research Area Signaling Transduction kit
Title Download Link
ARG80834 Human Androstenedione ELISA Kit User's manual download icon Download