ARG80858

Human Androstenedione ELISA Kit

Human Androstenedione ELISA Kit for ELISA and Human

Signaling Transduction kit

Overview

Product Description ARG80858 Human Androstenedione ELISA Kit provides competitive immunoenzymatic colorimetric method for the quantification of Androstenedione concentration in saliva.
Tested Reactivity Hu
Tested Application ELISA
Target Name Androstenedione
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 5 pg/ml
Sample Type Saliva.
Standard Range 20 - 1000 pg/ml
Sample Volume 50 µl

Application Instructions

Assay Time 1 h (37°C), 15 min (RT)

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Androstenedione
Background Androstenedione (also known as 4-androstenedione) is a 19-carbon steroid hormone produced in the adrenal glands and the gonads as an intermediate step in the biochemical pathway that produces the androgen testosterone and the estrogens estrone and estradiol. It is the common precursor of male and female sex hormones. Some androstenedione is also secreted into the plasma, and may be converted in peripheral tissues to testosterone and estrogens.

Androstenedione originates either from the conversion of dehydroepiandrosterone or from 17-hydroxyprogesterone. It is further converted to either testosterone or estrone.

The production of adrenal androstenedione is governed by ACTH, while production of gonadal androstenedione is under control by gonadotropins. In premenopausal women the adrenal glands and ovaries each produce about half of the total androstendione (about 3 mg/day). After menopause androstenedione production is about halved, primarily due to the reduction of steroid secreted by the ovary. Nevertheless, androstenedione is the principal steroid produced by the postmenopausal ovary. Increased Androstenedione levels often are seen in PCOS.
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Research Area Signaling Transduction kit
Title Download Link
ARG80858 Human Androstenedione ELISA Kit User's manual download icon Download