ARG82384
Human Annexin A1 ELISA Kit
Human Annexin A1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82384-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82384-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82384-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82384-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82384-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82384-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82384-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82384-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82384-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82384-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82384-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82384 Human Annexin A1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Annexin A1 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Annexin A1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 100 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.6% Inter-Assay CV: 6.6% |
| Alternate Names | ANX1; Calpactin II; Phospholipase A2 inhibitory protein; Chromobindin-9; LPC1; Annexin-1; Annexin A1; Lipocortin I; Annexin I; p35; Calpactin-2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANXA1 |
| Gene Full Name | annexin A1 |
| Background | This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014] |
| Function | Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Cell projection, cilium. Cell membrane. Membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Basolateral cell membrane. Apical cell membrane. Lateral cell membrane. Secreted, extracellular space. Cell membrane; Peripheral membrane protein; Extracellular side. Secreted, exosome. Cytoplasmic vesicle, secretory vesicle lumen. Cell projection, phagocytic cup. Early endosome. Cytoplasmic vesicle membrane; Peripheral membrane protein. [UniProt] |
| Highlight | Related products: Annexin antibodies; Annexin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated by protein kinase C, EGFR and TRPM7 (PubMed:2457390, PubMed:15485879). Phosphorylated in response to EGF treatment (PubMed:2532504). Sumoylated. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82384 Human Annexin A1 ELISA Kit User manual |
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