ARG82384

Human Annexin A1 ELISA Kit

Human Annexin A1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82384-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82384-002 Standard 2 X 10 ng/vial 4°C
ARG82384-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82384-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82384-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82384-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82384-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82384-008 25X Wash buffer 20 ml 4°C
ARG82384-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82384-010 STOP solution 10 ml (Ready to use) 4°C
ARG82384-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82384 Human Annexin A1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Annexin A1 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name Annexin A1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 100 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.6%
Inter-Assay CV: 6.6%
Alternate Names ANX1; Calpactin II; Phospholipase A2 inhibitory protein; Chromobindin-9; LPC1; Annexin-1; Annexin A1; Lipocortin I; Annexin I; p35; Calpactin-2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 301 Human ANXA1

Swiss-port # P04083 Human Annexin A1

Gene Symbol ANXA1
Gene Full Name annexin A1
Background This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]
Function Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. [UniProt]
Cellular Localization Nucleus. Cytoplasm. Cell projection, cilium. Cell membrane. Membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Basolateral cell membrane. Apical cell membrane. Lateral cell membrane. Secreted, extracellular space. Cell membrane; Peripheral membrane protein; Extracellular side. Secreted, exosome. Cytoplasmic vesicle, secretory vesicle lumen. Cell projection, phagocytic cup. Early endosome. Cytoplasmic vesicle membrane; Peripheral membrane protein. [UniProt]
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PTM Phosphorylated by protein kinase C, EGFR and TRPM7 (PubMed:2457390, PubMed:15485879). Phosphorylated in response to EGF treatment (PubMed:2532504).

Sumoylated. [UniProt]
Title Download Link
ARG82384 Human Annexin A1 ELISA Kit User manual download icon Download