ARG81479

Human ApoJ / Clusterin ELISA Kit

Human ApoJ / Clusterin ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81479-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81479-002 Standard 2 X 50 ng/vial 4°C
ARG81479-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81479-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81479-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81479-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81479-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81479-008 25X Wash buffer 20 ml 4°C
ARG81479-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81479-010 STOP solution 10 ml (Ready to use) 4°C
ARG81479-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81479 Human ApoJ / Clusterin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ApoJ / Clusterin in serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name ApoJ / Clusterin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%
Inter-Assay CV: 5.9%
Alternate Names Clusterin; Apolipoprotein J; SGP-2; SP-40; APOJ; ApoJbeta; CLU2; CLU1; NA1/NA2; TRPM2; CLI; SGP2; Apo-J; TRPM-2; ApoJalpha; Aging-associated gene 4 protein; APO-J; Complement cytolysis inhibitor a chain; Ku70-binding protein 1; Complement cytolysis inhibitor b chain; Testosterone-repressed prostate message 2; Complement-associated protein SP-40,40; KUB1; AAG4; Complement cytolysis inhibitor

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1191 Human CLU

Swiss-port # P10909 Human Clusterin

Gene Symbol CLU
Gene Full Name clusterin
Background The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]
Function Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. [UniProt]
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PTM Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.

Polyubiquitinated, leading to proteasomal degradation.

Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate. [UniProt]
Title Download Link
ARG81479 Human ApoJ / Clusterin ELISA Kit User's manual download icon Download