ARG80132

Human Apolipoprotein AI ELISA Kit

Human Apolipoprotein AI ELISA Kit for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Metabolism kit; Neuroscience kit; Signaling Transduction kit
publication_link Publication1

Component

Cat No Component Name Package Temp
ARG80132-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80132-002 Standard (Lyophilized) 3 X 40 ng/vial 4°C
ARG80132-003 Standard diluent buffer 20 ml 4°C
ARG80132-004 Antibody conjugate concentrate 400 µl 4°C
ARG80132-005 Antibody diluent buffer 16 ml 4°C
ARG80132-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80132-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80132-008 20X Wash buffer 50 ml 4°C
ARG80132-009 TMB substrate 12ml 4°C (Protect from light)
ARG80132-010 STOP solution 12ml 4°C
ARG80132-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80132 Human Apolipoprotein AI ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Apolipoprotein AI in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human ApoB, ApoC1, ApoC2, ApoC3, ApoD, ApoE, ApoE3, ApoER2, ApoH, ApoJ, ApoM
Target Name Apolipoprotein AI
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 0.6 ng/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 1.25 - 80 ng/ml
Sample Volume 100 µl
Precision

CV: <9%

Alternate Names ApoA-I; ProapoA-I; Apo-AI; Apolipoprotein A-I; Apolipoprotein A1; 1-242

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol APOA1
Gene Full Name apolipoprotein A-I
Background This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]
Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. [UniProt]
Highlight Related products:
Apolipoprotein antibodies; Apolipoprotein ELISA Kits;
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Simplify the ELISA analysis by GainData
Research Area Cancer kit; Cell Biology and Cellular Response kit; Metabolism kit; Neuroscience kit; Signaling Transduction kit
PTM Glycosylated.
Palmitoylated.
Phosphorylation sites are present in the extracellular medium.

Specific References

A colorimetric immunosensor based on self-linkable dual-nanozyme for ultrasensitive bladder cancer diagnosis and prognosis monitoring.

ELISA / Human

Peng Chen et al.
Biosens Bioelectron.,  (2018)

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Title Download Link
ARG80132 Human Apolipoprotein AI ELISA Kit User's manual download icon Download