ARG82744

Human Arylsulfatase A ELISA Kit

Human Arylsulfatase A ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82744-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82744-002 Standard 2 X 10 ng/vial 4°C
ARG82744-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82744-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82744-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82744-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82744-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82744-008 25X Wash buffer 20 ml 4°C
ARG82744-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82744-010 STOP solution 10 ml (Ready to use) 4°C
ARG82744-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82744 Human Arylsulfatase A ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Arylsulfatase A in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name Arylsulfatase A
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.6%
Inter-Assay CV: 5.9%
Alternate Names ASA; Cerebroside-sulfatase; EC 3.1.6.8; Arylsulfatase A; MLD

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 410 Human ARSA

Swiss-port # P15289 Human Arylsulfatase A

Gene Symbol ARSA
Gene Full Name arylsulfatase A
Background The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2010]
Function Hydrolyzes cerebroside sulfate. [UniProt]
Cellular Localization Lysosome. [UniProt]
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PTM The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD). [UniProt]
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