ARG82745

Human Arylsulfatase B ELISA Kit

Human Arylsulfatase B ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82745-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82745-002 Standard 2 X 10 ng/vial 4°C
ARG82745-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82745-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82745-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82745-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82745-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82745-008 25X Wash buffer 20 ml 4°C
ARG82745-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82745-010 STOP solution 10 ml (Ready to use) 4°C
ARG82745-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82745 Human Arylsulfatase B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Arylsulfatase B in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name Arylsulfatase B
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 32 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.2%
Inter-Assay CV: 5.8%
Alternate Names N-acetylgalactosamine-4-sulfatase; EC 3.1.6.12; MPS6; ASB; G4S; Arylsulfatase B

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 411 Human ARSB

Swiss-port # P15848 Human Arylsulfatase B

Gene Symbol ARSB
Gene Full Name arylsulfatase B
Background Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Removes sulfate groups from chondroitin-4-sulfate (C4S) and regulates its degradation. Involved in the regulation of cell adhesion, cell migration and invasion in colonic epithelium. In the central nervous system, is a regulator of neurite outgrowth and neuronal plasticity, acting through the control of sulfate glycosaminoglycans and neurocan levels (By similarity). [UniProt]
Cellular Localization Lysosome. Cell surface. [UniProt]
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PTM The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD). [UniProt]
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