ARG70086
Human BAFF recombinant protein (Active) (His-tagged, C-ter)
Human BAFF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human BAFF recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | BAFF |
| Species | Human |
| A.A. Sequence | MAVQG PEETV TQDCL QLIAD SETPT IQKGS YTFVP WLLSF KRGSA LEEKE NKILV KETGY FFIYG QVLYT DKTYA MGHLI QRKKV HVFGD ELSLV TLFRC IQNMP ETLPN NSCYS AGIAK LEEGD ELQLA IPREN AQISL DGDVT FFGAL KLL with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 0.5 ng/ml. |
| Alternate Names | BLYS; TALL1; Dendritic cell-derived TNF-like molecule; THANK; DTL; TNF- and APOL-related leukocyte expressed ligand 1; TNFSF20; CD257; BLyS; Tumor necrosis factor ligand superfamily member 13B; ZTNF4; CD antigen CD257; B lymphocyte stimulator; TALL-1; BAFF; B-cell-activating factor |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | TNFSF13B |
|---|---|
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 13b |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011] |
| Function | Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. Isoform 3: Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor ligand superfamily member 13b, soluble form: Secreted. [UniProt] |
| PTM | The soluble form derives from the membrane form by proteolytic processing. Isoform 2 is not efficiently shed from the membrane unlike isoform 1. N-glycosylated. [UniProt] |
