ARG80902

Human BD-4 / beta Defensin-4 ELISA kit

Human BD-4 / beta Defensin-4 ELISA kit for ELISA and Human

Immune System kit; Microbiology and Infectious Disease kit

Overview

Product Description

ARG80902 Human BD-4 / beta Defensin-4 ELISA kit is a Enzyme Immunoassay kit for the quantification of Human BD-4 / beta Defensin-4 in Serum, Plasma, Cell culture supernatants

Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with BD-1 (36 aa), BD-1 (47 aa), BD-2, BD-3.
Target Name BD4 / beta Defensin 4
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 0.8 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 1.56 - 100 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names DEFB-4; hBD-4; Defensin, beta 104; Beta-defensin 104; DEFB4; BD-4; Beta-defensin 4; DEFB104

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol DEFB104A
Gene Full Name defensin, beta 104A
Background Defensins are 2-6 kDa, cationic, microbicidal peptides active against many Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses [PMID: 8528769], containing three pairs of intramolecular disulphide bonds. On the basis of their size and pattern of disulphide bonding, mammalian defensins are classified into alpha, beta and theta categories. Every mammalian species explored thus far has beta-defensins. In cows, as many as 13 beta-defensins exist in neutrophils. However, in other species, beta-defensins are more often produced by epithelial cells lining various organs (e.g. the epidermis, bronchial tree and genitourinary tract).

Defensins are produced constitutively and/or in response to microbial products or proinflammatory cytokines. Some defensins are also called corticostatins (CS) because they inhibit corticotropin-stimulated corticosteroid production. The mechanism(s) by which microorganisms are killed and/or inactivated by defensins is not understood completely. However, it is generally believed that killing is a consequence of disruption of the microbial membrane. The polar topology of defensins, with spatially separated charged and hydrophobic regions, allows them to insert themselves into the phospholipid membranes so that their hydrophobic regions are buried within the lipid membrane interior and their charged (mostly cationic) regions interact with anionic phospholipid head groups and water. Subsequently, some defensins can aggregate to form `channel-like' pores; others might bind to and cover the microbial membrane in a `carpet-like' manner. The net outcome is the disruption of membrane integrity and function, which ultimately leads to the lysis of microorganisms. Some defensins are synthesised as propeptides which may be relevant to this process. [provide by Interpro: IPR006080]
Function Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103. [UniProt]
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Research Area Immune System kit; Microbiology and Infectious Disease kit
Title Download Link
ARG80902 Human BD-4 / beta Defensin-4 ELISA kit User Manual download icon Download