ARG70141
Human BDNF recombinant protein (Active) (His-tagged, C-ter)
Human BDNF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human BDNF recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | BDNF |
| Species | Human |
| A.A. Sequence | MHSDP ARRGE LSVCD SISEW VTAAD KKTAV DMSGG TVTVL EKVPV SKGQL KQYFY ETKCN PMGYT KEGCR GIDKR HWNSQ CRTTQ SYVRA LTMDS KKRIG WRFIR IDTSC VCTLT IKRGR with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce proliferation in BaF3 mouse pro-B cells transfected with TrkB. The ED50 for this effect is < 2 ng/ml. |
| Alternate Names | Brain-derived neurotrophic factor; Abrineurin; BULN2; ANON2; BDNF |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | 20 mM Sodium citrate (pH 3.5) and 0.2 M NaCl. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | BDNF |
|---|---|
| Gene Full Name | brain-derived neurotrophic factor |
| Background | The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009] |
| Function | During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: BDNF antibodies; BDNF ELISA Kits; BDNF recombinant proteins; Related news: The role of HDGF in tumor angiogenesis |
| PTM | The propeptide is N-glycosylated and glycosulfated. Converted into mature BDNF by plasmin (PLG). [UniProt] |
