ARG70101
Human BMP15 recombinant protein (Active) (His-tagged, C-ter)
Human BMP15 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human BMP15 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | BMP15 |
| Species | Human |
| A.A. Sequence | MAPLA TRQGK RPSKN LKARC SRKAL HVNFK DMGWD DWIIA PLEYE AFHCE GLCEF PLRSH LEPTN HAVIQ TLMNS MDPES TPPTC CVPTR LSPIS ILFID SANNV VYKQY EDMVV ESCGC R with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 17 ng/ml. |
| Alternate Names | POF4; ODG2; Growth/differentiation factor 9B; Bone morphogenetic protein 15; BMP-15; GDF9B; GDF-9B |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | 20 mM Sodium citrate (pH 3.5) and 0.2 M NaCl. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | BMP15 |
|---|---|
| Gene Full Name | bone morphogenetic protein 15 |
| Background | The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The transforming growth factor-beta superfamily includes large families of growth and differentiation factors. It is thought that this protein may be involved in oocyte maturation and follicular development as a homodimer or by forming heterodimers with a related protein, Gdf9. Defects in this gene are the cause of ovarian dysgenesis 2.[provided by RefSeq, Sep 2009] |
| Function | May be involved in follicular development. Oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
