ARG70102
Human BMP16 recombinant protein (Active) (His-tagged, C-ter)
Human BMP16 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human BMP16 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | BMP16 |
| Species | Human |
| A.A. Sequence | MHHLP DRSQL CRKVK FQVDF NLIGW GSWII YPKQY NAYRC EGECP NPVGE EFHPT NHAYI QSLLK RYQPH RVPST CCAPV KTKPL SMLYV DNGRV LLDHH KDMIV EECGC L with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 2.2 ng/ml. |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | 20 mM Sodium citrate (pH 3.5) and 0.2 M NaCl. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
