ARG70088
Human BMP3 recombinant protein (Active) (His-tagged, C-ter)
Human BMP3 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human BMP3 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | BMP3 |
| Species | Human |
| A.A. Sequence | MQWIE PRNCA RRYLK VDFAD IGWSE WIISP KSFDA YYCSG ACQFP MPKSL KPSNH ATIQS IVRAV GVVPG IPEPC CVPEK MSSLS ILFFD ENKNV VLKVY PNMTV ESCAC R with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 9.5 ng/ml. |
| Alternate Names | BMP-3; Bone morphogenetic protein 3; Osteogenin; BMP-3A; Bone morphogenetic protein 3A |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | 20 mM Sodium citrate (pH 3.5) and 0.2 M NaCl. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | BMP3 |
|---|---|
| Gene Full Name | bone morphogenetic protein 3 |
| Background | BMP3 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein, also known as osteogenin, induces bone formation. [provided by RefSeq, Jul 2008] |
| Function | Negatively regulates bone density. Antagonizes the ability of certain osteogenic BMPs to induce osteoprogenitor differentitation and ossification. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
