ARG70092
Human BMP7 recombinant protein (Active) (His-tagged, C-ter)
Human BMP7 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Publication2
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human BMP7 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | BMP7 |
| Species | Human |
| A.A. Sequence | MANVA ENSSS DQRQA CKKHE LYVSF RDLGW QDWII APEGY AAYYC EGECA FPLNS YMNAT NHAIV QTLVH FINPE TVPKP CCAPT QLNAI SVLYF DDSSN VILKK YRNMV VRACG CH with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 0.65 μg/ml. |
| Alternate Names | BMP-7; OP-1; Osteogenic protein 1; Eptotermin alfa; Bone morphogenetic protein 7 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | 20 mM Sodium citrate (pH 3.5) and 0.2 M NaCl. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | BMP7 |
|---|---|
| Gene Full Name | bone morphogenetic protein 7 |
| Background | The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. [provided by RefSeq, Jul 2008] |
| Function | Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| PTM | Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms. [UniProt] |
Specific References
BMP-７によるヒト歯根膜線維芽細胞のセメント芽細胞への分化制御
Functional assay / Human
舌癌におけるコリントランスポーターの局在および増殖細胞との関連
Functional assay / Human
