ARG70093
Human BMP8a recombinant protein (Active) (His-tagged, C-ter)
Human BMP8a recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human BMP8a recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | BMP8a |
| Species | Human |
| A.A. Sequence | MAVRP LRRRQ PKKSN ELPQA NRLPG IFDDV HGSHG RQVCR RHELY VSFQD LGWLD WVIAP QGYSA YYCEG ECSFP LDSCM NATNH AILQS LVHLM KPNAV PKACC APTKL SATSV LYYDS SNNVI LRKHR NMVVK ACGCH with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is 10-19.4 ng/ml. |
| Alternate Names | BMP-8A; Bone morphogenetic protein 8A |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | 20 mM Sodium citrate (pH 3.5) and 0.2 M NaCl. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | BMP8A |
|---|---|
| Gene Full Name | bone morphogenetic protein 8a |
| Function | Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis (By similarity). Signaling protein involved in regulation of thermogenesis and energy balance. Proposed to increase the peripheral response of brown adipose tissue (BAT) to adrenergic stimulation while acting centrally in the hypothalamus to increase sympathetic output to BAT. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
