ARG70094
Human BMP8b recombinant protein (Active) (His-tagged, N-ter)
Human BMP8b recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human BMP8b recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | BMP8b |
| Species | Human |
| A.A. Sequence | AVRPL RRRQP KKSNE LPQAN RLPGI FDDVH GSHGR QVCRR HELYV SFQDL GWLDW VIAPQ GYSAY YCEGE CSFPL DSCMN ATNHA ILQSL VHLMM PDAVP KACCA PTKLS ATSVL YYDSS NNVIL RKHRN MVVKA CGCH with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 21.8 ng/ml. |
| Alternate Names | BMP-8B; OP2; BMP-8; Osteogenic protein 2; Bone morphogenetic protein 8B; OP-2; BMP8 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | 20 mM Sodium citrate (pH 3.5) and 0.2 M NaCl. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | BMP8B |
|---|---|
| Gene Full Name | bone morphogenetic protein 8b |
| Background | The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has led to speculation of possible bone inductive activity. [provided by RefSeq, Jul 2008] |
| Function | Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis (By similarity). [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
