ARG70095

Human BMP9 recombinant protein (Active) (His-tagged, N-ter)

Human BMP9 recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (N-ter) Active Human BMP9 recombinant protein
Tested Application SDS-PAGE
Target Name BMP9
Species Human
A.A. Sequence SAGAG SHCQK TSLRV NFEDI GWDSW IIAPK EYEAY ECKGG CFFPL ADDVT PTKHA IVQTL VHLKF PTKVG KACCV PTKLS PISVL YKDDM GVPTL KYHYE GMSVA ECGCR with polyhistidine tag at the N-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 0.4 ng/ml.
Alternate Names GDF-2; BMP-9; Growth/differentiation factor 2; BMP9; Bone morphogenetic protein 9; HHT5

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer 20 mM Sodium citrate (pH 3.5) and 0.2 M NaCl.
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol GDF2
Gene Full Name growth differentiation factor 2
Background The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia. [provided by RefSeq, Jan 2014]
Function Potent circulating inhibitor of angiogenesis. Could be involved in bone formation. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG. [UniProt]
Cellular Localization Secreted. [UniProt]