ARG70292
Human BTN1A1 recombinant protein (Active) (ECD) (Fc-His-tagged, C-ter)
Human BTN1A1 recombinant protein (Active) (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Functional study and Human
Overview
| Product Description | HEK293 expressed, Fc-His-tagged (C-ter) Active Human BTN1A1 recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FuncSt, SDS-PAGE |
| Target Name | BTN1A1 (ECD) |
| Species | Human |
| A.A. Sequence | Ala27 - Arg242 of Human BTN1A1 (NP_001723.2) with an Fc-6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Activity | Active |
| Activity Note | Measured by its ability to inhibit Anti-CD3-induced proliferation of Jurkat Human acute T lymphocyte leukemia cells. The ED50 for this effect is 0.13-0.52 ng/ml. |
| Alternate Names | BT; BTN; Butyrophilin subfamily 1 member A1; BTN1 |
Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 1.0 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 97% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13410 Human Butyrophilin subfamily 1 member A1 |
|---|---|
| Gene Symbol | BTN1A1 |
| Gene Full Name | butyrophilin, subfamily 1, member A1 |
| Background | Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families [provided by RefSeq, Jul 2008] |
| Function | May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane (By similarity). Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion (By similarity). [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Secreted. [UniProt] |
| Calculated MW | 59 kDa |
| PTM | N-glycosylated. [UniProt] |
