ARG70292

Human BTN1A1 recombinant protein (Active) (ECD) (Fc-His-tagged, C-ter)

Human BTN1A1 recombinant protein (Active) (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Functional study and Human

Overview

Product Description HEK293 expressed, Fc-His-tagged (C-ter) Active Human BTN1A1 recombinant protein (ECD).
Tested Reactivity Hu
Tested Application FuncSt, SDS-PAGE
Target Name BTN1A1 (ECD)
Species Human
A.A. Sequence Ala27 - Arg242 of Human BTN1A1 (NP_001723.2) with an Fc-6X His tag at the C-terminus.
Expression System HEK293
Activity Active
Activity Note Measured by its ability to inhibit Anti-CD3-induced proliferation of Jurkat Human acute T lymphocyte leukemia cells. The ED50 for this effect is 0.13-0.52 ng/ml.
Alternate Names BT; BTN; Butyrophilin subfamily 1 member A1; BTN1

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 1.0 EU/µg of the protein, as determined by the LAL test.
Purity > 97% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 696 Human BTN1A1

Swiss-port # Q13410 Human Butyrophilin subfamily 1 member A1

Gene Symbol BTN1A1
Gene Full Name butyrophilin, subfamily 1, member A1
Background Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families [provided by RefSeq, Jul 2008]
Function May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane (By similarity). Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion (By similarity). [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. Secreted. [UniProt]
Calculated MW 59 kDa
PTM N-glycosylated. [UniProt]