ARG82650
Human C1q / Complement 1q ELISA Kit
Human C1q / Complement 1q ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82650 Human C1q / Complement 1q ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human C1q / Complement 1q in serum, plasma, cell culture supernatants, urine, saliva, milk and CSF. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Cross-Reactivity: Monkey: < 85% Dog: < 35% Mouse, Rat, Pig, Bovine and Rabbit: None No significant cross-reactivity observed with complement C1r, C1s, C2, C3, C4, C5, C6, C7, C8, C9, factor B, factor D, factor H, factor I and factor P. |
| Target Name | C1q / Complement 1q |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.097 ng/ml |
| Sample Type | Serum, plasma, cell culture supernatants, urine, saliva, milk and CSF. |
| Standard Range | 0.156 - 10 ng/ml |
| Sample Volume | 50 µl |
| Precision | Intra-Assay CV: 5.2% Inter-Assay CV: 10.0% |
| Alternate Names | C1q / Complement 1q subcomponent subunit A |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P02745 Human Complement C1q subcomponent subunit A |
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| Gene Symbol | C1QA |
| Gene Full Name | complement component 1, q subcomponent, A chain |
| Background | This gene encodes the A-chain polypeptide of serum complement subcomponent C1q, which associates with C1r and C1s to yield the first component of the serum complement system. C1q deficiency is associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains which include 6 A-chains, 6 B-chains, and 6 C-chains. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016] |
| Function | C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: Complement component antibodies; Complement component ELISA Kits; Complement component Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | O-linked glycans are assumed to be the Glc-Gal disaccharides typically found as secondary modifications of hydroxylated lysines in collagen-like domains. [UniProt] |
| Title | Download Link |
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| ARG82650 Human C1q / Complement 1q ELISA kit User manual |
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