ARG82360

Human CA2 / Carbonic Anhydrase 2 ELISA Kit

Human CA2 / Carbonic Anhydrase 2 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82360-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82360-002 Standard 2 X 50 ng/vial 4°C
ARG82360-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82360-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82360-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82360-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82360-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82360-008 25X Wash buffer 20 ml 4°C
ARG82360-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82360-010 STOP solution 10 ml (Ready to use) 4°C
ARG82360-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82360 Human CA2 / Carbonic Anhydrase 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CA2 / Carbonic Anhydrase 2 in serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine.
Tested Reactivity Hu
Tested Application ELISA
Target Name CA2 / Carbonic Anhydrase 2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.3%
Inter-Assay CV: 5.3%
Alternate Names Carbonic anhydrase C; EC 4.2.1.1; Car2; Carbonate dehydratase II; CA-II; Carbonic anhydrase II; CAC; HEL-76; Carbonic anhydrase 2; CAII

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 760 Human CA2

Swiss-port # P00918 Human Carbonic anhydrase 2

Gene Symbol CA2
Gene Full Name carbonic anhydrase II
Background The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
Function Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6. [UniProt]
Cellular Localization Cytoplasm. Cell membrane. Note=Colocalized with SLC26A6 at the surface of the cell membrane in order to form a bicarbonate transport metabolon. Displaced from the cytosolic surface of the cell membrane by PKC in phorbol myristate acetate (PMA)-induced cells. [UniProt]
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Title Download Link
ARG82360 Human CA2 / Carbonic Anhydrase 2 ELISA Kit User manual download icon Download