ARG82856

Human CA3 / Carbonic Anhydrase 3 ELISA Kit

Human CA3 / Carbonic Anhydrase 3 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82856-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82856-002 Standard 2 X 50 ng/vial 4°C
ARG82856-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82856-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82856-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82856-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82856-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82856-008 25X Wash buffer 20 ml 4°C
ARG82856-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82856-010 STOP solution 10 ml (Ready to use) 4°C
ARG82856-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82856 Human CA3 / Carbonic Anhydrase 3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CA3 / Carbonic Anhydrase 3 in serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine.
Tested Reactivity Hu
Tested Application ELISA
Target Name CA3 / Carbonic Anhydrase 3
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.9%
Inter-Assay CV: 5.5%
Alternate Names CAIII; EC 4.2.1.1; Car3; CA-III; Carbonate dehydratase III; Carbonic anhydrase 3; Carbonic anhydrase III

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 761 Human CA3

Swiss-port # P07451 Human Carbonic anhydrase 3

Gene Symbol CA3
Gene Full Name carbonic anhydrase III
Background Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]
Function Reversible hydration of carbon dioxide. [UniProt]
Cellular Localization Cytoplasm. [UniProt]
PTM S-glutathionylated in hepatocytes under oxidative stress. [UniProt]
Title Download Link
ARG82856 Human CA3 / Carbonic Anhydrase 3 ELISA Kit User manual download icon Download