ARG81461

Human CCL13 / MCP4 ELISA Kit

Human CCL13 / MCP4 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81461-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81461-002 Standard 2 X 10 ng/vial 4°C
ARG81461-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81461-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81461-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81461-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81461-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81461-008 25X Wash buffer 20 ml 4°C
ARG81461-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81461-010 STOP solution 10 ml (Ready to use) 4°C
ARG81461-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81461 Human CCL13 / MCP4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CCL13 / MCP4 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CCL13 / MCP4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 3.9 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 7.8 - 500 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.7%
Inter-Assay CV: 6.8%
Alternate Names SCYA13; C-C motif chemokine 13; Monocyte chemotactic protein 4; Small-inducible cytokine A13; CKb10; SCYL1; Monocyte chemoattractant protein 4; MCP-4; NCC-1; NCC1; CK-beta-10

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6357 Human CCL13

Swiss-port # Q99616 Human C-C motif chemokine 13

Gene Symbol CCL13
Gene Full Name chemokine (C-C motif) ligand 13
Background This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. This chemokine plays a role in accumulation of leukocytes during inflammation. It may also be involved in the recruitment of monocytes into the arterial wall during artherosclerosis. [provided by RefSeq, Sep 2014]
Function Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. [UniProt]
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PTM One major form (form long), and two minor forms (short chain and medium chain) are produced by differential signal peptide cleavage. The medium chain is about 30-fold less active than the long chain. [UniProt]
Title Download Link
ARG81461 Human CCL13 / MCP4 ELISA Kit User's manual download icon Download