ARG81678

Human CCL21 / Exodus 2 ELISA Kit

Human CCL21 / Exodus 2 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81678-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81678-002 Standard 2 X 10 ng/vial 4°C
ARG81678-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81678-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81678-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81678-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81678-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81678-008 25X Wash buffer 20 ml 4°C
ARG81678-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81678-010 STOP solution 10 ml (Ready to use) 4°C
ARG81678-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81678 Human CCL21 / Exodus 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CCL21 / Exodus 2 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CCL21 / Exodus 2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.2%; Inter-Assay CV: 5.8%
Alternate Names TCA4; Small-inducible cytokine A21; Beta-chemokine exodus-2; ECL; SCYA21; Secondary lymphoid-tissue chemokine; CKb9; 6Ckine; C-C motif chemokine 21; SLC

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6366 Human CCL21

Swiss-port # O00585 Human C-C motif chemokine 21

Gene Symbol CCL21
Gene Full Name chemokine (C-C motif) ligand 21
Background This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19). [provided by RefSeq, Sep 2014]
Function Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. [UniProt]
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Title Download Link
ARG81678 Human CCL21 / Exodus 2 ELISA Kit User's manual download icon Download